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At the end of all incubations, the hECs were washed three times with ice-cold PBS containing 0.1% BSA, once with acid stripping buffer (glycine 50 mM, NaCl 100 mM, pH 3) to remove the non-internalized, but cell surface-attached nanocarriers, and finally with PBS

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Recalculate, dont reuse a mark: If you change the reference dose or use a different bacteriostatic-water volume, recompute the unit count rather than reusing a fixed syringe mark

I drink maybe one glass of wine a month