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the lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA total protein assay, and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten minutes at 95 C

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The peptide selectively targets cells that have already undergone senescence as a consequence of earlier chemotherapy exposure, demonstrating a favorable safety profile for potential clinical application

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