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Mitochondrial dysfunction plays a key role in oocyte ageing and the decline in germ cell function, and an in-depth study of its molecular mechanisms and intervention strategies is highly important for slowing oocyte ageing, increasing fertility, and improving the success rate of assisted reproduction techniques

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Alassadi, M.J

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The recycling reaction was initiated by the addition of reaction buffer (100 mM sodium phosphate, pH 7.4), supplemented with 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) (100 M) (Sigma), NADPH (100 M) (Roth), glutathione reductase (1 U/ml) (Sigma), and EDTA (1 mM) (Sigma)