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When NNMT is overactive, it consumes S-adenosylmethionine (SAM) and reduces levels of nicotinamide adenine dinucleotide (NAD+), a critical coenzyme involved in cellular energy production

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K-means clustering analysis of amplicon sequence variants by animal demonstrated that NNMTi-treated DIO mice switched to LD had a distinct microbiome pattern that was highlighted by decreased Erysipelatoclostridium and increased Lactobacillus relative abundances compared to vehicle counterparts

Karabulut, A

Preclinical research evidence Animal Experiments Obese mouse model: After subcutaneous injection of AOD 9604 (250 g/kg) daily for 8 weeks, the body weight of obese mice decreased by 12%, the subcutaneous fat mass decreased by 25%, and the weight of epididymal adipose tissue decreased significantly