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Using HEK-293 adherent cells and K-562 suspension cells treated with varying concentrations of menadione or l-Buthionine Sulfoximine (BSO), two compounds known to affect total glutathione (GSH + GSSG) levels, we assayed the cells in separate assays and in a multiplex assay

After incubation, the fluorescence intensity of the biofilm was determined at Ex 544 nm and Em 590 nm (Tecan infinite M1000 pro, Sydney Australia)

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doi: 10.1210/en.2015-1546

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