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Functional analysis of Arabidopsis mutants points to novel roles for glutathione in coupling H 2 O 2 to activation of salicylic acid accumulation and signaling

Western blot analysis Cells were lysed in lysis buffer [50 mM Tris-HCl (pH 7.5), 1% NP-40, 0.5% (v/v) sodium deoxycholate, 150 mM NaCl and 1 Halt protease and phosphatase inhibitor mixture (Pierce)] on ice for 1 h

Step 2: Treatment Site Preparation The injection site is cleansed using medical-grade antiseptic protocols to maintain a sterile treatment environment

In addition, the activity of steroidogenic enzymes, such as P450scc, 3-HSD and 17-HSD

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