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Methods Animal experiments The humanized Townes SCD transgenic (B6;129- Hba tm1(HBA)Tow Hbb tm2(HBG1,HBB*)Tow / Hbb tm3(HBG1,HBB)Tow /J) mouse 65 was obtained from Jackson Laboratories ( as breeders

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After collection, the samples were left at room temperature for 60 min to complete the coagulation process, followed by centrifugation at 1300 g for 10 min to separate the serum

447 Conversely, MSI cancer cells with lower mtDNA copy numbers depended on glycolysis, which contributed to tumorigenesis and cisplatin chemoresistance