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Further, NOS activity and cytosolic protein content was evaluated in Sprague-Dawley rats at 0300, 0900, 1500, and 2100 h in various regions of the brain including cerebellum, brainstem, hypothalamus, and hippocampus and reported that a significant upregulation during the dark (active) period (Ayers et al., 1996)

Interspecies scaling (for example, body-surface-area conversion) typically reduces a rodent mg/kg figure severalfold, and even a properly scaled number would still be a hypothetical exposure , not a validated dose

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Blood pH levels should be between 7.35 to 7.45