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5-Ethynyl-2-deoxyuridine (EdU) incorporation assay DNA synthesis was assayed using the BeyoClick EdU Cell Proliferation Kit (Beyotime) according to the manufacturers instructions

typically 100300 mcg per administration in research contexts Administration: Intranasal spray

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This leads to a reduction in ovarian reserve and a higher occurrence of chromosomal abnormalities especially aneuploidy in oocytes 3,4 , increasing the risks of infertility, pregnancy loss, perinatal mortality, and congenital disorders in women at an advanced age 5,6