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Following the injection, in vivo imaging was conducted using an IVIS Spectrum (PerkinElmer) at various time points: 1 h, 2 h, 4 h, 6 h, 8 h, 12 h, 24 h, and 48 h post-injection, utilizing an excitation wavelength of 780 nm and a fluorescence emission wavelength of 840 nm

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Following infection of host cells, SARS-CoV-2 replicates and traffics its components to the ERGIC, where virions are assembled for subsequent release from the cell 3