The labeled cDNAs were used to detect marker genes of signal transduction pathways on microarray membranes using Human Signal Transduction PathwayFinder RT 2 Profiler PCR Array (SuperArray System, Bioscience, Frederick, MD, USA)
Once reconstituted with bacteriostatic water, AHK-Cu maintains full potency for 28 days at 28C refrigeration
Acid pH- or Zn 2+ -induced forms of this protein were classified as pre-molten globules characterized by a dramatic decrease in R g (from 37.8 0.9 at neutral pH to 27.6 0.9 at acidic pH or 28.1 0.8 at neutral pH in the presence of Zn 2+ ) and lack of globular structure (based on the absence of a bell-shaped peak in the corresponding Kratky plots) (Uversky et al., 1999
(2024), DOI - 10.3390/nu16193262,
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