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Enzymes and inhibitors Deacetylation experiments were carried out using recombinantly expressed and affinity purified human sirtuin deacetylases SIRT1 (aa 225-664, N-terminal His 6 -tag), SIRT2 (aa 50-356, with N-terminal GST-tag or N-terminal His 6 -tag), SIRT3 (aa 118-399), SIRT5 (aa 34-302) and commercially obtained SIRT6 (full length, C-terminal His 6 -tag, Sigma Aldrich, cat no