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10.1038/sigtrans.2017.23 98 LiuY.NiuR.YangF.YanY.LiangS.SunY.et al (2018)

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Making glutathione elution buffer isnt hard, but there are a few additional things to consider relative to elution buffers for other types of affinity purifications: To make glutathione elution buffer, add reduced glutathione powder to your buffer at a final concentration of 10 to 20 millimolar (mM) within an hour or two of when you will use the buffer

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Half-lives range from sub-day (liraglutide) to multi-day (semaglutide, tirzepatide), and molecular weight ranges from ~3.5 kDa (liraglutide, cagrilintide) to ~4.8 kDa (tirzepatide)