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However, we found that it is virtually fully oxidized, when expressed in the periplasm, suggesting that its engineered cysteine residues are completely oxidized by the oxidative folding machinery

[DOI] [PMC free article] [PubMed] [Google Scholar] 48.Rock W., Rosenblat M., Borochov-Neori H., Volkova N., Judeinstein S., Elias M., Aviram M

It was suggested that the instigator of the molecular and proteomic modifications includes an increase in the production of ROS, elevated stress proteins levels and higher levels of redox and inflammatory proteins

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In instances where the concentration of DOX exceeded the maximum value of the calibration curve, samples were diluted with 0.01 M HCl solution to ensure accurate quantification