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[55] If nervous system damage is present and blood testing is inconclusive, a lumbar puncture may be carried out to measure cerebrospinal fluid B 12 levels

Transwell assay The chemotactic motility of HUVECs was determined using transwell migration chambers (Corning) with 6.5 mm diameter polycarbonate filters (8 m pore size), as described previously.28 In brief, the bottom chambers were filled with 750 mL of RPMI 1640 medium containing all supplements

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