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The membrane was then blocked with 5% skim milk and subsequently incubated with primary antibodies for NLRP3 (1:1,000), ASC (1:1,000) and caspase-1 antibodies (1:500) overnight at 4C

To maintain freshness and moisture, wrap the ends in a damp paper towel, then place them in a plastic bag and store them upright in the fridge in 2 inches of water, if possible