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Furthermore, much of the current knowledge is based on preclinical studies, making it difficult to extrapolate these findings to humans due to inherent differences in disease manifestation, progression, and individual biological responses

The pain kept coming back every time I exercised, and my knee would swell and feel stiff for days

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The assay measures the increase in absorbance monitored at 340 nm associated with the formation of NADPH, which is proportional to the aconitase activity