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In contrast, a reduced risk for breast cancer was observed in blind women, with potentially higher levels of melatonin throughout all day (although there is no evidence that blind people produce more melatonin than sighted people) However, the mechanisms by which melatonin exerts any of oncostatic effects remains to be established

After three PBS washes, cells were blocked with 0.5% BSA in PBS, and incubated with the anti-SLC25A39 (Proteintech, 14693-1-AP, 1:200) and anti-ATP5A (Thermo Fisher Scientific, 439800, 1:200) primary antibodies in blocking solution for one hour