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Fluorescence polarization anisotropy assay Fluorescence polarization assays were conducted using a Cytation 5 cell imaging multi-mode reader (BioTek) equipped with a green FP filter at 25 C, utilizing 96-well microplates (SPL 30496)

Closing note Thoughtful use, open communication and routine monitoring keep B12 injections useful and safe

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We found that the number AgRP neurons positive for c-Fos was smaller in fasted AgRP GHR KO mice as compared to fasted control mice, while the number of non-AgRP cells positive for c-Fos remained unchanged between the groups (Fig