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coli cells (100 mL) were collected (6000 rpm, 4 C, 10 min) and resuspended in 5 mL of lysis buffer containing 8 M urea, 4 % (w/v) CHAPS, and 1 % (w/v) DTT and sonicated on ice for 90 cycles (a working period of 3 s in a 6 s interval for each cycle) at a power output of 200 W by an ultrasonic disruptor (JY92-II, Scientz Biotechnology Co., Ningbo, China)

However, calling it the active-B12 test is slightly misleading, because it measures all B12 bound to transcobalaminincluding biologically inactive forms

It will lead to fewer fine lines and wrinkles

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It also acts as a key regulator for several important physiological processes like photosynthesis and photorespiration, senescence ( Figure 1 )