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There's also growing interest in understanding individual variation in GHK-Cu responsewhy some people show dramatic improvements while others see more modest benefits

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Cells treated with test compounds were washed with PBS and incubated with 10 M 2,7-dichlorofluorescein diacetate (DCFH-DA) at 37 C for 30 min, followed by washing with PBS, digestion with trypsin, and collection

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Higher doses dont necessarily improve results and may increase the risk of side effects