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Separation was conducted at a constant temperature of 30 C using a mobile phase of 5% (v/v) acetonitrile, 0.05% (v/v) trifluoroacetic acid, and 0.1 M sodium perchlorate at a flow rate of 0.5 mL/min

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TEM imaging confirmed the uniform morphology of the nanoparticles that are spherical, while DLS analysis showed a particle size of 248.53 16.25 nm with a PDI of 0.274 and a zeta potential of 2.63 0.74 mV, confirming the colloidal stability