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Two pathways, the glutaredoxin and thioredoxin pathways, function in parallel, with the thioredoxins reduced by thioredoxin reductase (trxB) and the glutaredoxins reduced by glutathione, which is maintained in its reduced state by glutathione reductase (gor)

Moreover, the results of the transcriptomics analysis provide a molecular basis for the differences observed between the two differentiation methods, offer new insight into gene regulation in hepatogenesis in vitro and suggest ways to further improve hepatocyte differentiation in order to obtain more mature HLCs from human pluripotent stem cells for biomedical applications

As noted above, this is expected and aligns with the protocols design