First, to identify the clusters, the deconvolved (5 iterations) and bleached corrected images were filtered using a combination of Laplacian filter (3 pixels), to enhance their contrast, and the Gaussian filter (1 pixel), to reduce the background
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Anti-inflammantory and antioxidant effects of vitamin A (212, 213), C (214, 215), D (216, 217) and E (213) also involve inhibition of NF-kB as well as activation of Nrf-2 signaling pathways involved in cellular antioxidant response (218, 219)