The plates were agitated gently to mix the MTT into the media and then returned to the incubator for 2 hours
The MichaelisMenten constant (KM value) was previously determined to establish the substrate concentration at which the reaction rate reaches half of the maximum velocity (29)
Hofstetter W, Correa AM, Bekele N, Ajani JA, Phan A, Komaki RR, Liao Z, Maru D, Wu TT, Mehran RJ, Rice DC, Roth JA, Vaporciyan AA, Walsh GL, Francis A, Blackmon S, Swisher SG
The impact of instant coffee and decaffeinated coffee on the gut microbiota and depression-like behaviors of sleep-deprived rats
Unlike hormone replacement therapy (HRT) or testosterone replacement therapy (TRT), BPC-157 is not considered a hormone and does not directly replace testosterone