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[DOI] [PMC free article] [PubMed] [Google Scholar] 188.Kantlehner M, Schaffner P, Finsinger D, Meyer J, Jonczyk A, Diefenbach B, et al

Recording of the APEX2 Soret peak (absorbance at 406 nm) from a mixture of APEX2 (5 M) and H 2 O 2 (10 M), to which GSH (1 mM) and Na 2 S 4 (10 M) were added (right panel)

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For instance, in mouse embryonic stem cells, targeted disruption of glucose-6-phosphate dehydrogenase, which is the rate-limiting enzyme in the pentose phosphate pathway, the predominant metabolic pathway for the generation of NADPH (a cofactor of GR), renders cells extremely sensitive to H 2 O 2 -induced cell death 34