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2, Supplemental Material 1, Figure S1) was registered at www.clinicaltrials.gov under number NCT04946526 and approved by the Institutional Review Board of Appalachian State University (protocol number # 21-02216) where all interventions were conducted and samples were collected

Pre-mixed liquid vials are already reconstituted at manufacture and have a shorter usable window (1421 days) because oxidation begins immediately

A bottle used randomly once or twice a week is unlikely to give the same results as consistent daily use

If your vial will be used more than once over days or weeks, bacteriostatic water is required

Collectively, these features may have reduced the precision of cross-arm estimates and increased the probability of type II error