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As a superficial tumor, the causes of death in patients with breast cancer are reported to be due to cancer metastasis and/or relapse, which are significantly associated with the favorable tumor microenvironment [4]

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The antibiotic-resistant gene was removed from the genomic DNA of W303-1b/F using the Cre- loxP system, then W303-1b/F cells were further transformed with the linearized recombinant plasmid pGAPg-gshF to obtain engineered strain W303-1b/FF (Table 2)