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Second, GLP-1 secretion by engineered bacteria does not recapitulate the physiologically pulsatile release pattern of native L-cells, which depends on nutrient-derived stimuli, Ca 2+ signaling, and coordinated enteroendocrine regulation ( 10 Conclusion Current evidence indicates that the functional heterogeneity and adaptive potential of -cells in T2D are shaped by both intrinsic molecular programs and extrinsic regulatory cues, among which signals originating from the gut microbiota are particularly prominent

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