Plants have to adjust their metabolism in order to supply enough glutathione for AITC neutralization and in the same time to provide enough redox buffer for the nucleus to maintain proper cell proliferation
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The PCR conditions were adjusted according to the kit protocol as follows: reverse transcription for 10 min at 45 C, Polymerase activation for 2 min at 95 C, denaturation (40 cycles) for 5 s at 95 C, and annealing/extension for 20 s at 60 C
Phase II needs amino acids and other nutrients, including methionine, cysteine, glutamine, glycine, taurine, magnesium , glutathione, alpha-ketoglutarate, vitamins B5 and B12 , vitamin C, folate, and choline
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