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Tan IIA corrects the abnormal autophagic flux induced by DOX through a dual mechanism involving enhanced autophagosome formation and autolysosomal degradation, thereby improving cardiomyocyte viability (53)

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Real-time qPCR Total RNA was isolated with TRIZOL reagent (Invitrogen) and reverse transcription was performed by ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO), according to the manufacturers protocol