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A total of 150 ng of RNA from each sample was used in RT-PCRs performed in a total volume of 25 L using the QIAGEN OneStep RT-PCR Kit (Qiagen, Venlo, The Netherlands)

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GLOW Peptide Storage & Handling Storage Reference (All Three Compounds) State Lyophilized (Powder Form) BPC-157 -4 F (-20 C) long-term TB-500 -4 F (-20 C) long-term GHK-Cu -4 F (-20 C) long-term State Reconstituted (Liquid Form) BPC-157 35.6-46.4 F (2-8 C), up to 30 days TB-500 35.6-46.4 F (2-8 C), 1-2 weeks GHK-Cu 35.6-46.4 F (2-8 C), up to 30 days State Appearance BPC-157 Clear TB-500 Clear GHK-Cu May be light blue/green State Freeze reconstituted BPC-157 Aliquots possible TB-500 Do not freeze GHK-Cu Do not freeze TB-500 has the shortest reconstituted-stability window