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Association of Low Molecular Weight Plasma Aminothiols with the Severity of Coronavirus Disease 2019

Zheng, X., Ren, X.-M., Zhao, L

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Primers were designed to fit in resulting vector, pJB-HTS upstream of the hexa-histadine tag (5/phos/CCATGGGCAGCAGCCATCATCAT), and downstream (AGCTGGAATTCCTAGTTATTGCTCAGCGGTGGC) (Integrated DNA technologies) of the spacer yielding a fragment containing a phosphorylated 5 end, an initiation codon, a hexa-histadine tag, a thrombin cleavable sequence, a spacer, a termination codon, and an EcoRI site

The whole situation becomes a real Catch-22 situation