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Published 2021 May 1

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Cracking: 200mL of cracking reagent is prepared as TFA EDT TIS H 2 And (3) adding the full protection peptide under the ice-bath condition, returning to the room temperature after 0.5h, continuing to react for 2h, and adding anhydrous ether for precipitation after the reaction is finished

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Palmer CD, Scallan CD, Kraemer Tardif LD, Kachura MA, Rappaport AR, Koralek DO, et al