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Protein expression in BL21 was induced with 0.4 mM IPTG when the cultures reached an OD 600 of 0.60.8 at 37 C in LB medium and then incubated for 20 h at 20 C after induction

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A basic melting point apparatus for the analysis of crystalline solids consists of an oil bath with a transparent window (most basic design: a Thiele tube) and a simple magnifier

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