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Theoretical considerations regarding cell proliferation effects should be considered

Primary antibodies used for detecting shelterin subunits were custom rabbit anti-TRF1 (1:5,000, Thermo Scientific), mouse anti-TRF2 antibody (1:10,000, Abcam ab13579) and mouse anti-TIN2 antibody (1:10,000, Novus Biologicals 59B388.1)

NNMT inhibition supports improved metabolism and energy balance

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Storage Instructions : Reconstituted peptides must be stored in the refrigerator, dont freeze them in liquid form Non-reconstituted peptides can be kept at room temperature short-term, but refrigeration is preferred For long-term storage, freezing is acceptable only if the peptide is not reconstituted Avoid heat, direct sunlight, and repeated freeze-thaw cycles Disclaimer : These protocols are based on experimental and anecdotal use