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A 20 l mix, containing 8 l of cDNA sample, 10 l of SYBR Green PCR Master Mix (Thermo Fisher Scientific), and 2 l of 10 mM forward primer and reverse primer mixture was used to performed Quantitative RT-PCR with a StepOne Plus Real-time PCR system (Applied Biosystems, Foster City, CA, United States)

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For IL-1 experiments, THP-1 cells (1 10 6 per well) were seeded in a 12-well plate and differentiated overnight with 10 nM phorbol myristate acetate