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After three washes with TBST, membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Jackson Immunoresearch, Pennsylvania, USA) for 1.5 h at room temperature

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It is characterized by the release of small portions of Ca 2+ and ROS from the mitochondria [184], prompting a short-term depolarization of the IMM that activates the ETC and inhibits ROS production by the mitochondria [185]

Here are some tips to help you enjoy the benefits: Always conduct a patch test before applying to prevent any allergic reactions