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white arrow indicates normal neuron) and hippokampal region CA1, right (control (HC), more neurons damage (black arrow indicates ballonized neuron, white arrow indicates normal neuron)

Materials and methods Drugs and chemicals Powdered L-PAM and BSO (DL buthionine-( S,R )-sulfoximine) were purchased from Sigma-Aldrich (St Louis, MO, USA) and clinical grade BSO (L-buthionine ( S,R )-sulfoximine (50 mg/ml)) was provided by the National Cancer Institute (Bethesda, MD, USA)

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Youve learned the importance of a sterile environment and precise measurements to ensure the integrity of the peptide