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Membranes were incubated overnight at 4 C with Cleaved Caspase-3 (Asp175) (Cell Signalling Technology) and with peroxidase-linked anti-rabbit IgG or anti-mouse IgG secondary antibodies (GE Healthcare Life science) for 1 h at room temperature

Several studies found improved healing, reduced rates of leakage, and better tissue integrity at anastomotic sites in BPC-157-treated animals, findings with practical implications for surgical gastroenterology where anastomotic failure remains a serious complication

This analysis was conducted as an important assessment for the carrier to ensure it has minimal biotoxicity

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