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, GRX1-roGFP2 or TRaQ-G) enable real-time, organelle-specific redox readouts but primarily report redox potential rather than absolute GSH levels, whereas fractionation coupled with mass spectrometry provides absolute GSH/GSSG quantification but remains static and susceptible to lysis-associated artifacts

A375 cells were cultured until 3050% confluence, then treated with a complete growth medium containing 0.1 M doxorubicin (Sigma D1515)

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