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LCMS metabolomic analyses In vitro assay for succinate or glutarate release Experiments were performed as for the DTNB assay, but without the addition of 0.2 mM DTNB reagent

Recent prospectively designed studies have found higher WC increases CVD risk in older adults [27, 28], whilst meta-analyses demonstrate increased risk of incident dementia following obesity in mid-life, but a reduced risk when obesity is developed in older adulthood [29, 30]

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