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Astrocytes may be involved in the maintenance of BBB integrity and help restore a vessel when leaky since implantation of cultured astrocytes into normally leaky vessels results in tightening of the endothelium [45]

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10 ul of each drug solution was added to 90 ul of the rat blood serum samples and at specific time intervals the researchers terminated metabolism by adding 1 ml of ACN and acetic acid in order to precipitate the proteins which were then removed after overnight refrigeration followed by centrifugation

10.1016/j.bbrc.2017.08.093 210 YamashitaM.AokiH.HashitaT.IwaoT.MatsunagaT