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Clinical Update 2/07/2018 Does Protein Hurt Bones

To induce superovulation, mice were injected intraperitoneally with 10 IU of pregnant mare serum gonadotropin (PMSG), followed 48 hours later by 10 IU of human chorionic gonadotropin (hCG)

Kinetic fluorescence measurements were performed in a microplate reader at an excitation wavelength (ex) of 389 nm and an emission wavelength (em) of 513 nm, with a runtime of 15 min at 1-min intervals and a temperature set to 37 C to determine the average velocity (Vmean) of the enzymatic reaction

Subjective efficacy The investigator and the subjects subjectively evaluated the skin benefits of the GSSG and placebo lotions in accordance with a scoring scale (see Materials and Methods)

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