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After complete dissolution of the gel, the sample was added to a Qiagen spin column which was then centrifuged at 9200 g for 1 min

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The levels of NOX1 and NCF2 were also higher in the NETs-positive group compared to the NETs-negative group

Results Disruption of the glucagon receptor significantly alters both gene expression and the interactive network of cells To comprehensively examine the functional and physiological changes in cells following GCGR blockade, we conducted single-cell RNA sequencing (scRNA-seq) on islets isolated from 1214-week-old male wild-type (WT) and GCGR-knockout (KO) mice (Fig