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Semighini CP, Hornby JM, Dumitru R, Nickerson KW, Harris SD

(It may increase appetite, which some see as a bonus for bulking!)

Proteins for human CYP1A2, CYP3A4 or EGFR (1 g) were incubated with 10 g of FLAG-PGRMC1 (a.a.44195) treated with or without 50 mol l 1 haemin in 500 l of binding buffer containing 20 mmol l 1 HEPES-NaOH (pH 7.9), 100 mmol l 1 NaCl, 0.2 mmol l 1 EDTA, 10% glycerol and 0.1% NP40 for 60 min at room temperature

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From my perspective, one of the most important technical aspects of working with copper peptides is maintaining the appropriate pH range within the formulation