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Prior to sample loading the C18 disk was prepared by sequential treatment with methanol (20 L), acetonitrile/water/TFA (solvent B, 20 L, 80/20/0.1, v/v/v) and finally with solvent A (20 L)

In addition, the incidence increases with advancing age, peaking at 13.5% among individuals aged 65 years

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In these studies, researchers assembled a de novo transcriptome of 66,370 contigs from eight tissue libraries exposed to abiotic stress

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