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Nevertheless, the levels of protein produced by the TMV-Gate replicons is much higher than the amount of GFP reported 11 to be produced by conventional non-TMV based agroinfiltration vectors (0.030.04 mg GFP g 1 FW), full-length TMV genome based vectors (1.51.7 mg GFP g 1 FW) or pEAQ series of Cowpea mosaic virus-derived binary vectors (0.71.25 mg GFP g 1 FW) 33

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