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Another study developed pH/ROS dual-responsive metformin-released-enhanced self-healing bifunctional polyethylene glycol-co-poly(glycerol sebacic acid)/dihydrocaffeic acid and L-arginine co-grafted chitosan hydrogel to enhance wound healing through decreased inflammation and enhanced angiogenesis in a rat type II diabetic model

Serum homogenate was centrifuged at 21,000 g for 5 minutes at 4 C

According to the manufacturers protocols of the 8-OHdG ELISA KIT, the absorbance of the sample at 450 nm was measured on Microplate Reader (VICTOR3, PerkinElmer, Finland), and the concentration was calculated

Its primary limitation is a short systemic half-life of approximately 30 minutes, which necessitates frequent topical application or encapsulation technologies

doi: 10.1158/0008-5472.CAN-08-2287 57 BalsasPPalomeroJEguileorARodriguezMLVeglianteMCPlanas-RigolEet al