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The 5-lipoxygenase (5-LOX) inhibitor zileuton reduces inflammation and infarct size with improvement in neurological outcome following cerebral ischemia

Studied extensively in animal models for soft-tissue healing, tendon and ligament injury, and gastrointestinal mucosal protection

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The reaction was stopped by the addition of chloroform and methanol (2:1, v/v) followed by vortexing for 2 minutes

Rat blood serum samples were pre- equilibrated at 37C and then (50g) of each drug solution was added to 90 of rat serum in 1 .5mL Eppendorf tube maintained at 37C